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B047 |
VEILLONELLA AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Sodium thioglycollate Basic fuchsin Agar |
5.00 3.00 0.75 0.002 15.00 |
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Final pH (at 25°C) : 7.5 + 0.2 |
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Directions |
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Suspend 24 gms. in 1000ml distilled water containing 21ml of 60%, sodium lactate. If desired, 1g of polysorbate 80 may be added. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 50-55°C and aseptically add Vancomycin to a final concentration of 7.5 mcg/ml medium. |
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