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B1124 |
FLUID THIOGLYCOLLATE MEDIUM W/O AGAR |
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Formula |
B1124 |
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Ingredients |
gm/lit. |
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Casein enzymic hydrolysate Yeast extract Dextrose Sodium chloride L-Cystine Sodium thioglycollate Resazurin |
15.00 5.00 5.50 2.50 0.50 0.50 0.001 |
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Final pH (at 25°C) : |
7.1 + 0.2 |
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Directions |
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Suspend 29 gm. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. Cool to 25° C and store in a cool dark place preferably below 25°C. Note : If more than the upper one third has acquired the pink colour, the medium may be restored once by heating in a water bath or in free flowing steam until the pink colour disappears |
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