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B119 |
BRAIN HEART INFUSION AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Calf brain,infusion from Beef infusion from Proteose peptone Dextrose Sodium chloride Disodium phosphate Agar |
200.00 250.00 10.00 2.00 5.00 2.50 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 52 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before pouring. If desired,20 units Penicillin and 40 microgram Streptomycin per ml of medium may be added to make the medium selective for fungi. |
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