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B318 |
SHAPTON MEDIUM |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Casein enzymic hydrolysate Beef extract Yeast extract Dextrose Bromo cresol purple Agar |
5.00 2.50 3.00 1.00 1.00 0.025 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 27.53 gms. in 1000ml. distilled water. Soak for 5 minutes. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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