|
B377 |
ROGOSA SL BROTH |
|||
|
Formula |
B377 |
|||
|
Ingredients |
gms/lit. |
|||
|
Casein enzymic hydrolysate Yeast extract Dextrose Arabinose Saccharose Sodium acetate Ammonium citrate Monopotassium phosphate Magnesium sulphate Manganese sulphate Ferrous sulphate Polysorbate 80 |
10.00 5.00 10.00 5.00 5.00 15.00 2.00 6.00 0.57 0.12 0.03 1.00 |
|||
|
Final pH (at 25°C) : 5.4 + 0.2 |
||||
|
Directions |
||||
|
Suspend 60 gms. of B377 in 1000ml. distilled water. Boil to dissolve the medium completely. Add 1.32 ml glacial acetic acid. Mix thoroughly, distribute into culture tubes or flasks. Heat to 90-100°C for 2-3 minutes. Cool to 45°C for direct inoculation. DO NOT AUTOCLAVE. |
||||