B469 

CLOSTRIDIAL AGAR

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Papaic digest of soyabean meal 

Dextrose 

Sodium chloride 

Sodium thioglycollate 

L-Cystine 

Sodium formaldehyde sulphoxylate 

Neomycin sulphate 

Sodium azide 

Agar 

17.00

3.00

6.00

2.50

1.80

0.25

1.00

0.15

0.20

14.50

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 46 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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