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B493 |
PE-2 MEDIUM |
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Formula |
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Ingredients |
gm/lit. |
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Peptic digest of animal tissue Yeast extract Bromo cresol purple |
20.00 3.00 0.04 |
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Final pH (at 25°C) : Self |
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Directions |
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Suspend 23 gm. in 1000ml distilled water. Heat if necessary to dissolve the medium completely. Dispense 18-20 ml aliquots into 18 x 150 mm screw capped test tubes. Add 8-10 untreated Alaska seed peas and let the tubes stand for 1 hour to effect hydration. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. |
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