B559 

INORGANIC SALT STARCH AGAR (ISP MEDIUM NO.4)

Formula

Ingredients

gms/lit.

Starch soluble

Dipotassium phosphate

Magnesium sulphate

Sodium chloride

Ammonium sulphate

Calcium carbonate

Ferrous sulphate

Manganous chloride

Zinc sulphate

Agar

10.00

1.00

1.00

1.00

2.00

2.000

0.001

0.001

0.001

20.000

Final pH (at 25°C) : 7.2 + 0.2

Directions

Suspend 37 gms in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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