B606 

MAINTENANCE MEDIUM FOR B. SUBTILIS ATCC 6633

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Casein enzymic hydrolysate

Yeast extract

Beef extract

Dextrose

Agar

6.00

4.00

3.00

1.50

1.00

15.00

Final pH (at 25°C) : 6.6 + 0.2

Directions 

Suspend 30.5 gms in 1000ml distilled water. Heat to boiling to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes.

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