B705 

SBG ENRICHMENT BROTH

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Yeast extract

Mannitol

Sodium taurocholate

Sodium hydrogen selenite

Dipotassium phosphate

Monopotassium phosphate

Brilliant green

5.00

5.00

5.00

1.00

4.00

2.65

1.02

0.005

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 23.7 gms. in 1000ml. distilled water. Heat to boiling for 5-10 minutes. DO NOT AUTOCLAVE OR OVERHEAT. Dispense in sterile tubes. Add 0.5 g/l Sodium sulfapyridine if desired. 

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