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B734 |
STREPTOCOCCUS AGALACTIAE SELECTIVE AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Meat extract Sodium chloride Esculin Thallous sulphate Crystal violet Agar |
10.00 5.00 5.00 1.00 0.333 0.0013 13.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 34.34 gms. in 940ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C and add 60ml. defibrinated blood and 25 ml. Staphylococcus β toxin. Mix well and pour into sterile petri plates. |
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