B829 

YEAST MANNITOL BROTH

Formula 

B829

Ingredients 

gms/lit.

Yeast extract

Mannitol

Dipotassium phosphate

Magnesium sulphate

Sodium chloride

Calcium carbonate

1.00

10.00

0.50

0.20

0.10

1.00

Final pH (at 25°C) : 6.8 + 0.2

Directions 

Suspend 12.8 gms. of (B829) in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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