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B977 |
CHARCOAL BLOOD AGAR BASE |
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Formula |
B977 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Yeast extract Sodium chloride Starch, soluble Charcoal Agar |
10.00 10.00 3.50 5.00 10.00 4.00 12.00 |
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Final pH (at 25°C) : |
7.5 + 0.2 |
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Directions |
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Suspend 54.5 gms. of B977 in 1000 ml. distilled water. Boil to dissolve the medium with frequent stirring. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Add 10 ml of sterile defibrinated horse blood, 0.3 ml of sterile 100 ug/ml Penicillin solution and 0.3 ml of 0.1% solution of 4:4 diamidodiphenylamine hydrochloride per 100 ml of Charcoal Blood Agar Base (B977). |
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